Spermidine/spermine-N-acetyltransferase ablation protects against liver and kidney ischemia-reperfusion injury in mice
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چکیده
Kamyar Zahedi, Alex B. Lentsch, Tomohisa Okaya, Sharon Barone, Nozomu Sakai, David P. Witte, Lois J. Arend, Leena Alhonen, Jason Jell, Juhani Jänne, Carl W. Porter, and Manoocher Soleimani Division of Nephrology and Hypertension and Department of Surgery, University of Cincinnati College of Medicine, Cincinnati; Division of Pathology, Children’s Hospital Medical Center, Cincinnati; Division of Pathology, University of Cincinnati College of Medicine, Cincinnati; A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland; Roswell Park Cancer Institute, Buffalo, New York; and Veterans Affairs Medical Center, Cincinnati, Ohio
منابع مشابه
Spermidine/spermine-N1-acetyltransferase ablation protects against liver and kidney ischemia-reperfusion injury in mice.
Expression of spermine/spermidine-N1-acetyltransferase (SSAT), the rate-limiting enzyme of polyamine backconversion cascade, increases after ischemia-reperfusion injuries (IRI). We hypothesized that SSAT plays an important role in the mediation of IRI. To test our hypothesis, wild-type (SSAT-wt) and SSAT-deficient (SSAT-ko) mice were subjected to liver or kidney IRI by ligation of hepatic or re...
متن کاملProximal Tubule Epithelial Cell Specific Ablation of the Spermidine/Spermine N1-Acetyltransferase Gene Reduces the Severity of Renal Ischemia/Reperfusion Injury
BACKGROUND Expression and activity of spermidine/spermine N1-acetyltransferase (SSAT) increases in kidneys subjected to ischemia/reperfusion (I/R) injury, while its ablation reduces the severity of such injuries. These results suggest that increased SSAT levels contribute to organ injury; however, the role of SSAT specifically expressed in proximal tubule epithelial cells, which are the primary...
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Polyamines (putrescine, spermidine, and spermine) are present in all higher eukaryotic cells and are essential for cell growth, differentiation and apoptosis. Sharing common precursor with polyamines, nitric oxide (NO) is associated with myocardial ischemia/reperfusion injury by the generation of peroxynitrite. Although polyamines have been implicated in tissue ischemia injury, their metabolism...
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Introduction: Preliminary studies have con.rmed reduction in cell death following treatment with antioxidants. According to this .nding we study the relationship between consumption of CoQ10 and expression of Bax and Bcl2 in hippocampus following ischemia/reperfusion as proteins involved in cell programmed death or apoptosis. Methods: We studied the protective role of CoQ10 against ischemia-rep...
متن کاملOverexpression of SSAT in kidney cells recapitulates various phenotypic aspects of kidney ischemia-reperfusion injury.
To ascertain the role of spermidine/spermine N-1-acetyl-transferase (SSAT; the rate-limiting enzyme in polyamine catabolism) in cell injury, cultured kidney (HEK 293) cells conditionally overexpressing SSAT were generated. The SSAT expression was induced and its enzymatic activity increased 24 h after addition of tetracycline and remained elevated over the length of the experiments. Induction o...
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تاریخ انتشار 2009